Microbiology Practice Test 5
Microbiology NCLEX Practice Test
Microbiology is a key topic within the NCLEX test plan, located under Nursing Science → Clinical Foundations → Microbiology. This section explains pathogens, host defenses, and antimicrobial stewardship essential for infection control. Each test contains 50 questions designed to mirror the difficulty and variety of the real exam.
This is the 5th part of the Microbiology series. To explore all practice tests under this topic, use the “Back to Main Topic” button at the end of the page.
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In the Microbiology Study Cards section, shared by real NCLEX candidates, you’ll find concise summaries and high-yield insights related to the most tested concepts. It’s a perfect space to reinforce challenging topics and sharpen your recall through quick, focused repetitions. Short, powerful, and repeatable!
Microbiology Practice Test 5
Gonorrhea is an STD caused by which type of organism?
- Virus
- Bacteria
- Parasite
- Fungus
Explanation: Answer reason: Gonorrhea is caused by the bacterium Neisseria gonorrhoeae, a Gram-negative diplococcus.
Which of the following conditions is often seen in patients with gonorrhea?
- Trachoma
- Chlamydia
- Herpes
- Syphilis
Explanation: Answer reason: Chlamydia commonly co-infects with Neisseria gonorrhoeae; co-testing and co-treatment are standard. Trachoma is ocular chlamydial disease, and herpes/syphilis are different STIs not specifically associated as frequent co-infections with gonorrhea.
What is this disease called?
- Jundice
- Measelse
- Maleria
- Tetanus
Explanation: Answer reason: Among the options, measles is the communicable disease name; 'jundice' is a symptom, and 'maleria' and 'tetanus' are different conditions.
Ringworm infects which part of the body?
- Liver
- Skin
- Eyes
- Intestine
Explanation: Answer reason: Ringworm (tinea) is a dermatophyte fungal infection that involves keratinized tissues, primarily the skin.
What is the most common cause of pneumonia?
- Influenza virus
- Streptococcus pneumoniae
- Staphylococcus aureus
- Mycoplasma pneumoniae
Explanation: Answer reason: Streptococcus pneumoniae (pneumococcus) is the most common cause of community-acquired pneumonia in adults.
Which of the following is a viral disease?
- Malaria
- Tuberculosis
- AIDS
- Typhoid
Explanation: Answer reason: AIDS is caused by the human immunodeficiency virus (HIV). Malaria is protozoal, tuberculosis is bacterial (Mycobacterium tuberculosis), and typhoid is bacterial (Salmonella typhi).
What is the mode of transmission of leprosy?
- Breast milk
- Insect bite
- Droplet infection
- Both insect bite and droplet infection
Explanation: Answer reason: Leprosy (Mycobacterium leprae) is primarily transmitted via prolonged close contact through nasal/oral respiratory droplets, not by insects or breast milk.
What is the chain of spread of malaria?
- Man-arthropod-man
- Pig-man-man
- Mammal-bird-man
- Bird-arthropod-man
Explanation: Answer reason: Human malaria is transmitted by the Anopheles mosquito (an arthropod) from human to human; thus the chain is man → arthropod → man. Other animal hosts listed are not part of human malaria transmission.
What is the first step in the control of communicable disease?
- Prevention of disease
- Treatments
- Notification
- Surveillance
Explanation: Answer reason: Reporting suspected or confirmed cases initiates surveillance and enables prompt control measures; thus notification is the first step.
What are spherical bacteria occurring in clusters called?
- Staphylo cocci
- Strepto cocci
- Pneumo cocci
- Coccobacilli
Explanation: Answer reason: Staphylococci are spherical bacteria arranged in grape-like clusters; streptococci form chains, coccobacilli are short rods, and 'pneumo' denotes a genus/site not a cluster arrangement.
Which of the following infections of animals can primarily be transmitted through milk?
- Tuberculosis
- Dengue
- Plague
- Leptospirosis
Explanation: Answer reason: Mycobacterium bovis from infected cattle is transmitted to humans via unpasteurized milk. Dengue is mosquito-borne, plague is flea/rodent-borne, and leptospirosis is usually via water contaminated with urine.
Which substance destroys or inhibits the growth of microorganisms?
- Disinfectant
- Antiseptic
- Bactericidal
Explanation: Answer reason: Antiseptics are agents applied to living tissue that inhibit the growth of or destroy microorganisms; disinfectants act on objects, and bactericidal refers specifically to killing bacteria.
What is the mode of transmission of hepatitis A?
- Sexual contact
- Contaminated food
- Blood transfusion
- Mosquito bite
Explanation: Answer reason: Hepatitis A is transmitted via the fecal–oral route, commonly through ingestion of contaminated food or water; it is not spread by blood transfusion or vectors like mosquitoes.
What is the diagnostic test used for scarlet fever?
- Mantoux test
- Dick test
- Schick test
- Widal test
Explanation: Answer reason: The Dick test assesses susceptibility to the erythrogenic toxin of Streptococcus pyogenes associated with scarlet fever. Mantoux is for TB, Schick for diphtheria, and Widal for typhoid.
What is the term for the constant presence of infection in a given geographical area without any external input?
- Epidemic
- Endemic
- Pandemic
- Sporadic
Explanation: Answer reason: Endemic describes the constant baseline presence of a disease or infectious agent within a specific geographic area without external introduction. Epidemic is a sudden increase, pandemic is worldwide spread, and sporadic is irregular infrequent cases.
Widal test is performed for identifying which microorganism?
- Clostridium
- Salmonella
- Staphylococcus
- Meningococcal
Explanation: Answer reason: The Widal test detects agglutinating antibodies (O and H) against Salmonella typhi/paratyphi, used in typhoid fever diagnosis.
Hepatitis is a what type of disease?
- Bacterial disease
- Viral disease
- Fungal disease
- Auto-immunity disease
Explanation: Answer reason: Most common forms of hepatitis (A, B, C, D, E) are caused by viruses, so it is classified as a viral disease.
What is the causative organism of cat scratch disease?
- Rota virus
- Hemorrhagic colitis
- Bartonella henselae
- Staphylococcus aureus
Explanation: Answer reason: Cat scratch disease is caused by the gram-negative bacillus Bartonella henselae; other options are not the etiologic agent.
Sterilization by using ionizing radiations is called as-?
- Pasteurization
- Ionization
- Cold sterilization
- Tyndallization
Explanation: Answer reason: Ionizing radiation (gamma rays, electron beams) sterilizes without significant heat, hence termed cold sterilization. Pasteurization and tyndallization are heat methods; 'ionization' is not the standard name of the process.
Candidiasis is a-?
- Bacterial disease
- Viral disease
- Fungal disease
- Protozal disease
Explanation: Answer reason: Candidiasis is caused by Candida species (yeasts), making it a fungal infection.
What is the most common cause of meningitis in children aged 6 months to 2 years?
- Meningococci
- Pneumococci
- Influenza
- Coli
Explanation: Answer reason: Haemophilus influenzae type b historically is the most common bacterial cause of meningitis in children 6 months to 2 years; E. coli is typical in neonates, while pneumococcus and meningococcus predominate in older children/adults.
Which stain is used to identify acid-fast bacilli?
- Giemsa
- Wright's
- Ziehl-Neelsen
- Leishman
Explanation: Answer reason: Acid-fast bacilli (e.g., Mycobacterium) are identified using the Ziehl-Neelsen acid-fast stain; the others are Romanowsky-type blood stains.
Which type of hepatitis is commonly associated with pregnancy and high mortality?
- Hepatitis A
- Hepatitis B
- Hepatitis C
- Hepatitis E
Explanation: Answer reason: Hepatitis E infection is notably severe in pregnancy, with high maternal mortality especially in the third trimester; other hepatitis types do not carry this characteristic risk.
Which of the following is NOT an example of a house fly borne disease?
- Diarrhoea
- Cholera
- Gastroenteritis
- Trench fever
Explanation: Answer reason: Houseflies mechanically transmit diarrheal diseases such as diarrhoea, cholera, and gastroenteritis. Trench fever is transmitted by the human body louse (Bartonella quintana), not by houseflies.
The registered nurse is conducting an in-service for colleagues on the subject of peptic ulcers. The nurse would be correct in identifying which of the following as a causative factor?
- N. gonorrhea
- Influenza
- Pylori
- Coli
Explanation: Answer reason: Helicobacter pylori infection is the primary infectious cause of peptic ulcer disease; the other organisms are unrelated to peptic ulcers.
Which of the following is NOT a sexually transmitted disease?
- Trichomoniasis
- Byssinosis
- Acquired immunodeficiency syndrome
- Syphilis
Explanation: Answer reason: Byssinosis is an occupational lung disease from inhaling cotton dust, not sexually transmitted. The others are sexually transmitted infections.
Teichoic acid is present in which type of bacteria?
- Gram negative bacteria
- Gram positive
- Both A & B
- None of above
Explanation: Answer reason: Teichoic acids are structural polymers of Gram-positive bacterial cell walls (including lipoteichoic acid); they are absent in Gram-negative bacteria.
Which disease affects the parotid gland?
- Measles
- Mumps
- Rubella
- Chickpox
Explanation: Answer reason: Mumps, a paramyxovirus infection, classically causes parotitis with swelling of the parotid salivary glands. Measles, rubella, and chickenpox do not specifically target the parotid gland.
What parasite is identified using a Scotch tape swab?
- Tape worm
- Pin worm
- Angilostoma
- Hook worm
Explanation: Answer reason: The Scotch tape test collects perianal eggs of Enterobius vermicularis (pinworm), making it the standard identification method.
What is the portal of entry for the chickenpox virus?
- Urinary tract
- Respiratory tract
- Faeco-oral route
- All
Explanation: Answer reason: Varicella-zoster virus typically enters via inhalation of airborne droplets, infecting the upper respiratory mucosa; thus the portal of entry is the respiratory tract.
What does a positive Schick test indicate?
- Immunity to diphtheria
- Susceptibility to diphtheria
- Hypersensitivity to diphtheria
- Infection with diphtheria
Explanation: Answer reason: A positive Schick skin test shows a local reaction because neutralizing antitoxin is absent, indicating susceptibility to diphtheria; a negative test indicates immunity.
What is the 'Klebs Loeffler bacillus' the name of?
- Streptococci
- Salmonella tumhi
- Cornybacterium
- Meningococci
Explanation: Answer reason: Klebs–Loeffler bacillus is the historical name for Corynebacterium diphtheriae.
A greenish yellow membrane over the pharynx, larynx, and tonsil is seen in which condition?
- Tonsillitis
- Yellow fever
- Diphtheria
- Henson disease
Explanation: Answer reason: Diphtheria causes a characteristic thick greenish-gray pseudomembrane over the pharynx, larynx, and tonsils due to Corynebacterium diphtheriae exotoxin.
The nurse is caring for a patient with cervical carcinoma most likely caused by which virus?
- Adenovirus
- Epstein-Barr virus
- Human papillomavirus
- Varicella zoster virus
Explanation: Answer reason: Cervical carcinoma is strongly associated with high-risk HPV types (especially 16 and 18). EBV is linked to nasopharyngeal carcinoma and Burkitt lymphoma; adenovirus and varicella zoster are not typical causes of cervical cancer.
Which of the following infectious agents can be transmitted by blood transfusion?
- Hepatitis
- HIV
- Plasmodium
- All of the above
Explanation: Answer reason: Hepatitis viruses (B, C), HIV, and Plasmodium (malaria) are known transfusion-transmitted infections, so all listed agents can be spread via blood transfusion.
Which type of tuberculosis (TB) is the most infectious?
- Spinal TB
- Menangis TB
- Intestine TB
- Pulmonary TB
Explanation: Answer reason: Pulmonary TB spreads via airborne droplets from the lungs and is the contagious form; extrapulmonary TB (spinal, intestinal, meningeal) is generally not infectious.
What test is done to diagnose diphtheria?
- Widal test
- Montoux test
- VDRL test
- Shick test
Explanation: Answer reason: The Schick test assesses susceptibility to diphtheria toxin and historically was used in diphtheria evaluation. Other options correspond to typhoid (Widal), tuberculosis (Mantoux), and syphilis (VDRL).
Which sterilization method is used for culture media?
- Autoclave
- Hot air oven
- Ethylene oxide
- Glutaraldehyde
Explanation: Answer reason: Culture media are routinely sterilized by moist heat in an autoclave (121°C, 15 psi). Dry heat (hot air oven) is for glassware, ethylene oxide and glutaraldehyde are for equipment and instruments, not media.
TCBS agar is used to isolate which organism?
- Cholera
- Mycobacterium
- Staphylococcus
- Diphtheria
Explanation: Answer reason: TCBS (Thiosulfate-Citrate-Bile Salts-Sucrose) agar is selective for Vibrio species, particularly Vibrio cholerae, which produces yellow colonies by sucrose fermentation.
From where did the coronavirus get its name?
- Due to their crown-like projections.
- Due to their leaf-like projections.
- Due to their surface structure of bricks.
- None of the above.
Explanation: Answer reason: Coronavirus is named for its crown-like spike projections on the virion surface; corona means crown.
What is the most common cause of diarrhea in a boy who suddenly develops diarrhea after being treated for fever in the hospital?
- Coli
- Stress
- Rota virus
- Antibiotics
Explanation: Answer reason: Hospitalized child treated for fever likely received antibiotics; sudden diarrhea is commonly antibiotic-associated (e.g., C. difficile).
What is the media of transmission of bovine type of tuberculosis bacillus?
- Milk.
- Water.
- Air.
- Food.
Explanation: Answer reason: Mycobacterium bovis (bovine TB) is commonly transmitted to humans through ingestion of unpasteurized milk and dairy products.
What is Coronavirus?
- It is a large family of viruses
- It belongs to the family of Nidovirus
- Both A and B are correct
- Only A is correct
Explanation: Answer reason: Coronaviruses are a large family of enveloped RNA viruses and are classified within the nidovirus group (order Nidovirales), so both statements A and B are true.
Which of the following diseases are related to coronavirus?
- MERS
- SARS
- Both A and B
- Neither A nor B
Explanation: Answer reason: Both MERS and SARS are caused by coronaviruses (MERS-CoV and SARS-CoV), so both are related to coronavirus.
Where was the first novel coronavirus case identified?
- Beijing
- Shanghai
- Wuhan, Hubei
- Tianjin
Explanation: Answer reason: Early confirmed cases of the 2019 novel coronavirus were first reported in Wuhan, Hubei, China.
What type of microorganism causes typhoid?
- Bacteria
- Fungi
- Virus
Explanation: Answer reason: Typhoid fever is caused by Salmonella Typhi, a gram-negative bacterium.
What is the term for an epidemic that spreads from country to country or over the whole world?
- Pandemic
- Zoonoses
- Sporadic
- Endemic
Explanation: Answer reason: A pandemic is an epidemic that spreads across multiple countries or worldwide. Zoonoses are animal-to-human diseases; sporadic means infrequent, isolated cases; endemic is constant presence in a region.
Kala Azar disease is related to which vector?
- Sand fly
- Mosquito
- Somt tick
- Tsetse fly
Explanation: Answer reason: Kala-azar (visceral leishmaniasis) is transmitted by sand flies (Phlebotomus spp.).
The agent-host-environment model of health and illness is based on the concept of which option?
- Stage of illness
- Infectious disease
- Prognosis
- Rate of Exposure
Explanation: Answer reason: The agent-host-environment (epidemiologic triad) explains disease occurrence through interactions among an infectious agent, a susceptible host, and the environment—i.e., the concept of infectious disease causation.
What term describes the lodgment, development, and reproduction of arthropods on the surface of the body or in clothing?
- Infection
- Infestation
- Contamination
- Pollution
Explanation: Answer reason: Arthropods such as lice and mites residing and multiplying on the body surface or clothing are ectoparasites, which is termed infestation. Infection implies invasion of tissues by microbes; contamination is mere presence; pollution refers to environmental impurities.
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