Biochemistry Practice Test 3
Biochemistry NCLEX Practice Test
Biochemistry is a key topic within the NCLEX test plan, located under Nursing Science → Clinical Foundations → Biochemistry. This section links metabolic processes to nutrition, medication action, and laboratory interpretation in nursing care. Each test contains 50 questions designed to mirror the difficulty and variety of the real exam.
This is the 3rd part of the Biochemistry series. To explore all practice tests under this topic, use the “Back to Main Topic” button at the end of the page.
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Biochemistry Practice Test 3
In presence of the following cofactor, pyruvate carboxylase converts pyruvate to oxaloacetate?
- ATP, Protein and CO2
- CO2 and ATP
- CO2
- Protein
Explanation: Answer reason: Pyruvate carboxylase requires ATP and uses CO2 (as bicarbonate), with biotin as the coenzyme. Of the options, the correct required pair is CO2 and ATP.
The carbohydrate reserved in human body is?
- Starch
- Glucose
- Glycogen
- Inulin
Explanation: Answer reason: Humans store carbohydrates as glycogen in liver and muscle. Starch and inulin are plant storage polysaccharides, and glucose is the circulating monosaccharide, not the storage form.
The distinguishing test between monosaccharides and disaccharide is.....?
- Bial’s test
- Seliwanoff’s test
- Barfoed’s test
- Hydrolysis test
Explanation: Answer reason: Barfoed’s test distinguishes monosaccharides from disaccharides by their ability to reduce copper(II) ions in acidic medium rapidly (monosaccharides react within ~2 minutes), whereas disaccharides react more slowly. Bial’s is for pentoses, Seliwanoff’s for ketoses vs aldoses, and hydrolysis is not the classic distinguishing test.
What is the cause of scurvy?
- Not being exposed to enough sunlight
- Misfolding protiens
- Defective collagen formation
- All of the above
Explanation: Answer reason: Scurvy results from vitamin C deficiency, which impairs proline/lysine hydroxylation in collagen synthesis, causing defective collagen formation.
Albumin is a type of __________ protein?
- Simple
- Conjugated
- Derived
- All of them
Explanation: Answer reason: Albumin is a simple globular protein without a nonprotein (prosthetic) group; therefore it is not conjugated or derived.
Proteins are held together by __________ bond?
- Glycoside
- Peptide
- Triple bond
- None of them
Explanation: Answer reason: Proteins are polymers of amino acids linked by peptide (amide) bonds between the carboxyl and amino groups. Glycosidic bonds join sugars; a triple bond is unrelated.
Esters of long chain fatty acids and long chain alcohols are called ____________?
- Fat
- Waxes
- Glycerol
- Hydrocarbon
Explanation: Answer reason: Waxes are esters formed from long‑chain fatty acids and long‑chain alcohols. Fats are triacylglycerols (fatty acids esterified to glycerol); glycerol and hydrocarbons are not esters.
LDL (Low density lipo protein) is also known as ____________ protein?
- Good
- Bad
- Moderate
- None of them
Explanation: Answer reason: LDL carries cholesterol to peripheral tissues and contributes to atherosclerosis, hence it is termed the "bad" lipoprotein.
The general formula of monosaccharides is?
- CnH2nOn
- C2nH2On
- CnH2O2n
- CnH2nO2n
Explanation: Answer reason: Monosaccharides have the empirical formula (CH2O)n, which corresponds to CnH2nOn.
A pentose sugar is?
- Dihydroxyacetone
- Ribulose
- Erythrose
- Glucose
Explanation: Answer reason: Ribulose is a five-carbon (pentose) ketose. Dihydroxyacetone is a triose, erythrose is a tetrose, and glucose is a hexose.
The sugar found in DNA is?
- Xylose
- Ribose
- Deoxyribose
- Ribulose
Explanation: Answer reason: DNA contains deoxyribose as its pentose sugar, whereas RNA contains ribose; xylose and ribulose are not components of nucleic acids.
The sugar found in RNA is?
- Ribose
- Deoxyribose
- Ribulose
- Erythrose
Explanation: Answer reason: RNA contains ribose sugar; DNA contains deoxyribose. Ribulose and erythrose are different sugars not found in RNA.
Starch is a?
- Polysaccharide
- Monosaccharide
- Disaccharide
- None of these
Explanation: Answer reason: Starch is a polymer of glucose units, classifying it as a polysaccharide.
An essential amino acid in man is?
- Aspartate
- Tyrosine
- Methionine
- Serine
Explanation: Answer reason: Methionine is an essential amino acid for humans; aspartate, tyrosine, and serine are nonessential (tyrosine is conditionally essential only when phenylalanine is limited).
The amino acid with a non polar side chain is?
- Serine
- Valine
- Asparagine
- Threonine
Explanation: Answer reason: Valine has a hydrophobic aliphatic side chain and is nonpolar, whereas serine, threonine, and asparagine have polar side chains (hydroxyl or amide groups).
Casein, the milk protein is?
- Nucleoprotein
- Chromoprotein
- Phosphoprotein
- Glycoprotein
Explanation: Answer reason: Casein is a phosphoprotein with phosphate groups esterified to serine residues, forming calcium caseinate micelles in milk.
The degradative processes are categorized under the heading of?
- Anabolism
- Catabolism
- Metabolism
- None of the above
Explanation: Answer reason: Degradative processes that break down molecules and often release energy are classified as catabolism; anabolism is biosynthetic, and metabolism includes both.
The monosaccharide units are linked by 1→α4 glycosidic linkage in?
- Maltose
- Sucrose
- Cellulose
- Cellobiose
Explanation: Answer reason: Maltose consists of two glucose units joined by an α-1,4 glycosidic bond. Sucrose is linked α1↔β2, while cellulose and cellobiose have β-1,4 linkages.
Mucopolysaccharides are?
- Hamopolysaccharides
- Hetropolysaccharides
- Proteins
- Amino acids
Explanation: Answer reason: Mucopolysaccharides (glycosaminoglycans) are heteropolysaccharides composed of repeating disaccharide units containing amino sugars and uronic acids.
All proteins contain the?
- Same 20 amino acids
- Different amino acids
- 300 Amino acids occurring in nature
- Only a few amino acids
Explanation: Answer reason: Proteins are polymers built from the same set of 20 standard amino acids, although many other amino acids exist in nature.
Tertiary structure of a protein describes?
- The order of amino acids
- Location of disulphide bonds
- Loop regions of proteins
- The ways of protein folding
Explanation: Answer reason: Tertiary structure refers to the overall three-dimensional folding of a single polypeptide chain; primary structure is the amino acid order. While disulfide bonds help stabilize tertiary structure and loops are local features, the best single description is the ways of protein folding.
Denaturation of proteins results in?
- Disruption of primary structure
- Breakdown of peptide bonds
- Destruction of hydrogen bonds
- Irreversible changes in the molecule
Explanation: Answer reason: Protein denaturation disrupts secondary/tertiary structure mainly by breaking hydrogen and other noncovalent bonds. The primary structure and peptide bonds remain intact, and denaturation may be reversible or irreversible; therefore the consistently correct result is destruction of hydrogen bonds.
The cholesterol molecule is?
- Benzene derivative
- Quinoline derivative
- Steroid
- Straight chain acid
Explanation: Answer reason: Cholesterol is a sterol based on the four-ring steroid nucleus (cyclopentanoperhydrophenanthrene), not a benzene or quinoline derivative nor a straight-chain acid.
Mucopolysaccharides are?
- Hampolysaccharides
- Hetropolysaccharides
- Proteins
- Amino acids
Explanation: Answer reason: Mucopolysaccharides (glycosaminoglycans) consist of repeating disaccharide units (amino sugar + uronic acid), making them heteropolysaccharides, not proteins, amino acids, or homopolysaccharides.
Which of the following is disaccharide?
- Glucose
- Lactose
- Sucrose
- Both B and C
Explanation: Answer reason: Lactose and sucrose are disaccharides; glucose is a monosaccharide.
........ catalyse the reaction of transfer of a group is?
- Transpeptidases
- Protease
- Methylases
- Arginases
Explanation: Answer reason: Methylases transfer a methyl group to a substrate, exemplifying transferase activity. Protease and arginase are hydrolases; transpeptidases are not the prototypical group-transfer example.
Protein portion of Enzyme is called ...........?
- Holoenzyme
- Apoenzyme
- Prosthetic group
- Allosteric group
Explanation: Answer reason: The protein component of an enzyme without its cofactor is the apoenzyme; holoenzyme = apoenzyme + cofactor; prosthetic group is the tightly bound cofactor.
Alpha helix is spring like structure in which 1st Amino Acid attached with ....... Amino acid?
- 3rd
- 4th
- 5th
- 2nd
Explanation: Answer reason: In an alpha helix, hydrogen bonding occurs between the carbonyl oxygen of residue i and the amide hydrogen of residue i+4; thus the first amino acid bonds with the fifth.
A sugar is called aldose when it contains __________ group?
- Ketone
- Aldehyde
- Carboxyl
- Amide
Explanation: Answer reason: Aldoses are carbohydrates whose carbonyl group is an aldehyde; ketoses contain a ketone. Carboxyl and amide groups do not define sugar classes.
The break down of complex molecule to simpler one is called ________?
- Anabolism
- Catabolism
- Both of them
- Non of them
Explanation: Answer reason: Catabolism is the metabolic breakdown of complex molecules into simpler ones; anabolism builds complex molecules.
Haemoglobin has?
- Primary structure
- Quaternery structure
- Secondary structure
- Tertiary structure
Explanation: Answer reason: Hemoglobin is a tetramer of four polypeptide subunits (2 α and 2 β), exhibiting quaternary protein structure.
In mammals, the major fat in adipose tissues is?
- Phospholipid
- Cholesterol
- Sphingolipids
- Triacylglycerol
Explanation: Answer reason: Adipocytes primarily store energy as triacylglycerols; phospholipids, cholesterol, and sphingolipids are mainly membrane components.
The pentose sugar present mainly in the heart muscle is?
- Lyxose
- Ribose
- Arabinose
- Xylose
Explanation: Answer reason: Lyxose is the pentose reported to be present predominantly in heart muscle; ribose is ubiquitous in nucleic acids, while arabinose and xylose are primarily plant sugars.
A simple protein found in the nucleoproteins of the sperm is?
- Prolamine
- Protamine
- Glutelin
- Globulin
Explanation: Answer reason: Protamine are simple, highly basic proteins that replace histones in sperm nuclei and are components of nucleoproteins.
Carr-Price reaction is used to detect?
- Vitamin A
- Vitamin D
- Ascorbic acid
- Vitamin E
Explanation: Answer reason: Carr-Price reaction (SbCl3 giving a blue color) is a qualitative test for vitamin A/carotenoids.
Example of hydrolases is?
- Amylases
- Aminotransferases
- Synthatase
- Glucokinase
Explanation: Answer reason: Hydrolases catalyze bond cleavage using water; amylases hydrolyze starch. The others are transferases/ligases (aminotransferases, synth(et)ase, kinase).
Amino Acid is building blocks of protein having charge depends upon on?
- N terminal
- C terminal
- R Group
- No of bonds
Explanation: Answer reason: The net charge of an amino acid at a given pH is determined primarily by the ionizable side chain (R group), which can be acidic, basic, or neutral.
________ contains hydroxyl group bonded to a carbon atom of Alkane chain?
- Alcohol
- Ether
- Amines
- Aldehydes
Explanation: Answer reason: An alcohol is an R–OH compound with a hydroxyl group attached to a saturated carbon atom. Ethers are R–O–R', amines contain an amino group (–NH2), and aldehydes have a –CHO group.
The two common polysaccharides are starch and ____________?
- Galactose
- Glucose
- Fructose
- Glycogen
Explanation: Answer reason: Glycogen is a common polysaccharide; the other choices are monosaccharides.
When two molecules of glucose are combined together is called __________?
- Lactose
- Sucrose
- Maltose
- Glucose
Explanation: Answer reason: Two glucose monomers combine via an alpha-1,4 glycosidic bond to form the disaccharide maltose.
The plasma membrane of a human cell is?
- A membrane that contains pili on its cell surface
- A double layer of carbohydrate enclosing the cell
- A single layered cell membrane
- A double layer of phospholipids
Explanation: Answer reason: Human plasma membranes are phospholipid bilayers. Pili occur in bacteria, not human cells; the membrane is not a carbohydrate bilayer nor a single layer.
Enzymes, which are produced in inactive form in the living cells, are called?
- Apoenzymes
- Lysozymes
- Papain
- Proenzymes
Explanation: Answer reason: Inactive precursor forms of enzymes are termed proenzymes (zymogens) and become active after specific proteolytic activation. Apoenzyme is the protein part of an enzyme, lysozyme and papain are specific enzymes.
An example of Phosphoprotein present in egg yolk is?
- Ovoalbumin
- Ovoglobulin
- Ovovitellin
- Avidin
Explanation: Answer reason: Ovovitellin is a phosphoprotein of egg yolk, whereas ovalbumin, ovoglobulin, and avidin are proteins of egg white and not phosphoproteins.
An example of a hydroxy fatty acid is?
- Ricinoleic acid
- Crotonic acid
- Butyric acid
- Oleic acid
Explanation: Answer reason: Ricinoleic acid is a 12-hydroxy fatty acid found in castor oil; the others lack a hydroxyl group.
The fatty acid present in cerebrosides is?
- Lignoceric acid
- Valeric acid
- Caprylic acid
- Behenic acid
Explanation: Answer reason: Cerebrosides (myelin glycosphingolipids) characteristically contain very long-chain saturated fatty acids such as lignoceric acid (and its 2‑hydroxy form). The other options are not typical.
Retinoic acid participates in the synthesis of?
- Iodopsin
- Rhodopsin
- Glycoprotein
- Cardiolipin
Explanation: Answer reason: Retinoic acid (vitamin A acid) regulates gene expression for epithelial differentiation and is essential for glycoprotein synthesis; visual pigments rhodopsin/iodopsin require retinal, not retinoic acid.
Niacin can be synthesized in human beings from?
- Histidine
- Phenylalanine
- Tyrosine
- Tryptophan
Explanation: Answer reason: Niacin (vitamin B3) is synthesized endogenously from the essential amino acid tryptophan via the kynurenine pathway; other listed amino acids are not precursors.
Niacin deficiency can occur in?
- Hartnup disease
- Phenylketonuria
- Alkaptonuria
- None of these
Explanation: Answer reason: Hartnup disease causes defective transport of neutral amino acids, including tryptophan, leading to reduced synthesis of niacin from tryptophan and a pellagra-like niacin deficiency.
The bond between two amino acid is ........?
- Glycosidic bond
- Peptide bond
- H-H bond
- Ester Bond
Explanation: Answer reason: Amino acids link via a peptide (amide) bond formed between the carboxyl group of one and the amino group of another. Glycosidic bonds join sugars; ester bonds are typical in lipids; H-H bond is irrelevant.
Enzyme catalyzed reaction in which transfer/addition of Hydrogen occur is?
- Transferases
- Reductases
- Oxidases
- Hydrotransferases
Explanation: Answer reason: Addition or transfer of hydrogen corresponds to reduction; enzymes catalyzing such reactions are reductases (oxidoreductases).
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