Biochemistry Practice Test 1
Biochemistry NCLEX Practice Test
Biochemistry, within the NCLEX test plan under Nursing Science → Clinical Foundations, reflects the core knowledge domains and conceptual competencies directly related to what the exam evaluates. The targeted number of questions is 50; designed with realistic clinical scenarios and conceptual variety to help you identify both your strengths and improvement areas.
This test is the 1st part of the Biochemistry section. To explore all practice tests under this topic, use the “Back to Main Topic” button at the end of the page.
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Biochemistry Practice Test 1
Which of the following is an example of a chromoprotein?
- Haemoglobin
- Mucin
- Glutenin
- Casein
Explanation: Answer reason: Hemoglobin is a chromoprotein because it contains a heme group with an iron-containing porphyrin ring that gives it color and allows it to bind oxygen. Chromoproteins are conjugated proteins with a colored prosthetic group, typically a pigment such as heme.
Number of carbons in tetroses are ________________?
- 2
- 4
- 6
- 8
Explanation: Answer reason: Tetroses are monosaccharides that contain four carbon atoms; the prefix “tetra-” means four, so the correct number of carbons in a tetrose is 4.
Polysaccharides are?
- Polymers
- Acids
- Proteins
- Oils
Explanation: Answer reason: Polysaccharides are long-chain carbohydrates composed of repeating monosaccharide units linked by glycosidic bonds, making them biological polymers such as starch, glycogen, and cellulose.
The number of isomers of glucose is?
- 2
- 4
- 8
- 16
Explanation: Answer reason: Glucose is an aldohexose with four chiral centers, and the number of possible stereoisomers is determined by the formula 2ⁿ, where n is the number of chiral carbons. Therefore, 2⁴ = 16 stereoisomers, making 16 the correct answer.
The protein present in hair is?
- Keratin
- Elastin
- Myosin
- Tropocollagen
Explanation: Answer reason: Keratin is the primary structural protein found in hair, composed of fibrous alpha-helical polypeptides rich in cysteine, which form strong disulfide bonds that provide rigidity and resilience.
Glucose is .....carbon compound?
- 5 c
- 6 c
- 7 c
- 4 c
Explanation: Answer reason: Glucose contains six carbon atoms, making it a hexose sugar. Its chemical formula is C₆H₁₂O₆, confirming that it is a 6-carbon compound.
Example of keto sugar is ………?
- Fructose
- Galactose
- Mannose
- glucose
Explanation: Answer reason: Fructose is a keto sugar because it contains a ketone functional group at the second carbon, classifying it as a ketohexose. In contrast, glucose, galactose, and mannose are aldo sugars with an aldehyde group.
The Symbol of Glutamate is?
- E
- Q
- G
- T
Explanation: Answer reason: The single-letter symbol for glutamate is E, while Q represents glutamine. The single-letter amino acid codes are standardized based on nomenclature and structural distinctions, making E the correct symbol for glutamate.
2nd shell has _________ numbers of electrons?
- 2
- 8
- 16
- 32
Explanation: Answer reason: The second electron shell (n=2) can hold a maximum of 8 electrons because it contains one 2s orbital (2 electrons) and three 2p orbitals (6 electrons), giving a total capacity of 8.
An example of chromoprotein is?
- Hemoglobin
- Sturine
- Nuclein
- Gliadin
Explanation: Answer reason: Hemoglobin is a chromoprotein because it contains a heme prosthetic group with an iron-containing porphyrin ring that imparts color and enables oxygen binding. Chromoproteins are conjugated proteins associated with a colored pigment such as heme.
The simplest amino acids are?
- Lysine
- Bile acids
- Glycine
- Alanine
Explanation: Answer reason: Glycine is the simplest amino acid because its side chain consists of a single hydrogen atom, making it the smallest and least structurally complex of all amino acids. This minimal structure allows greater flexibility within protein chains.
The sugar found in RNA is?
- Ribose
- Deoxyribose
- Ribulose
- Erythrose
Explanation: Answer reason: RNA contains D-ribose with a 2′-OH group; DNA contains 2′-deoxyribose. The other listed sugars are not constituents of RNA.
Net ATPs used in aerobic glycolysis are ______?
- 4
- 6
- 8
- 10
Explanation: Answer reason: In aerobic glycolysis, net substrate-level ATP gain is 2 and 2 NADH are formed. Using the classical P/O ratio (3 ATP per cytosolic NADH via shuttle), total ATP equivalents are 2 + 6 = 8
Uracil and ribose form?
- Adenosine
- Cytidine
- Guanosine
- Uridine
Explanation: Answer reason: A nucleoside is a base plus ribose. Pyrimidines take the -idine suffix; uracil + ribose is uridine. Adenosine and guanosine are purine nucleosides; cytidine is cytosine + ribose
The sugar found in DNA is?
- Xylose
- Ribose
- Deoxyribose
- Ribulose
Explanation: Answer reason: DNA contains deoxyribose as its pentose sugar; ribose is found in RNA, and xylose/ribulose are not DNA components.
Starch is a?
- Polysaccharide
- Monosaccharide
- Disaccharide
- None of these
Explanation: Answer reason: Starch consists of many glucose units (amylose and amylopectin) and is therefore a polysaccharide.
Casein, the milk protein is?
- Nucleoprotein
- Chromoprotein
- Phosphoprotein
- Glycoprotein
Explanation: Answer reason: Casein contains covalently bound phosphate groups and forms calcium–phosphate micelles; it is classified as a phosphoprotein
Cofactor is ....... part of holoenzyme?
- Organic
- Inorganic
- Carbohydrate
Explanation: Answer reason: A holoenzyme consists of an apoenzyme plus a non-protein component. The inorganic non-protein component (often a metal ion) is termed a cofactor; the organic non-protein component is a coenzyme.
The one alphabet symbol of Arginine is ......?
- W
- K
- Q
- R
Explanation: Answer reason: Arginine’s standard one-letter amino acid code is R; W is tryptophan, K is lysine, and Q is glutamine.
Tripeptide is a chain having ...... amino acid?
- 3
- 4
- 5
- 2
Explanation: Answer reason: A tripeptide consists of three amino acids linked by peptide bonds.
K is the symbol of .......?
- Keratin
- Lysine
- Leucine
- Glutamic Acid
Explanation: Answer reason: In standard one-letter amino acid codes, K denotes lysine. Leucine is L, and glutamic acid is E.
Each turn of α-helix contains the amino acid residues (number)?
- 3.6
- 3.0
- 4.2
- 4.5
Explanation: Answer reason: An α-helix has 3.6 amino acid residues per turn (pitch ~5.4 Å), making 3.6 the correct number.
F is the symbol of .......?
- Phenylalanine
- Methionine
- Tyrosine
- Histidine
Explanation: Answer reason: The one-letter amino acid code F denotes phenylalanine. Methionine is M, tyrosine is Y, and histidine is H.
The carbohydrate reserved in human body is?
- Starch
- Glucose
- Glycogen
- Inulin
Explanation: Answer reason: Humans store carbohydrates as glycogen in the liver and skeletal muscle. Starch and inulin are plant storage polysaccharides, and glucose is a circulating monosaccharide rather than a storage form.
Which of the following is polysaccharide?
- Insulin
- Agar
- Lactose
- Sucrose
Explanation: Answer reason: Agar is a complex polysaccharide from red algae; lactose and sucrose are disaccharides, and insulin is a peptide hormone.
Enzymes that catalyze electron transport are present mainly in the?
- Endoplasmic reticulum
- Inner mitochondrial membrane
- Lysosomes
- Ribosomes
Explanation: Answer reason: The electron transport chain complexes and ATP synthase are embedded in the inner mitochondrial membrane, where oxidative phosphorylation occurs.
An example of Aldo sugar is...?
- Fructose
- Glucose
- Chitin
- Cellulose
Explanation: Answer reason: An aldo sugar (aldose) contains an aldehyde group; glucose is an aldose. Fructose is a ketose, and chitin and cellulose are polysaccharides.
An example of a saturated fatty acid is?
- Palmitic acid
- Oleic acid
- Linoleic acid
- Erucic acid
Explanation: Answer reason: Palmitic acid is a fully saturated 16-carbon fatty acid; the others (oleic, linoleic, and erucic) are unsaturated.
Which of the following is a proteinaceous substance?
- Starch
- Amyloid
- Heparin
- Glycogen
Explanation: Answer reason: Starch is a polysaccharide (carbohydrate), not a protein. Note that heparin (a glycosaminoglycan) and glycogen (a polysaccharide) are also not proteins, whereas amyloid is proteinaceous.
A process in which an electron is lost is called __________?
- Oxidation
- Reduction
- Sublimation
- Condensation
Explanation: Answer reason: The loss of electrons is oxidation (OIL RIG: Oxidation Is Loss, Reduction Is Gain).
In which of the following cells does the Krebs cycle not occur?
- Neurons
- Type II alveolar cell
- Epithelial cell
- Red blood cells
Explanation: Answer reason: The Krebs (TCA) cycle occurs in the mitochondria. Mature red blood cells lack mitochondria, so they cannot perform the Krebs cycle and rely on anaerobic glycolysis. Neurons, type II alveolar cells, and epithelial cells contain mitochondria.
Which of the following polysaccharides is indigestible by man?
- Amylopectin
- Glycogen
- Amylose
- Cellulose
Explanation: Answer reason: Humans lack the enzyme cellulase to hydrolyze the beta-1,4-glycosidic bonds in cellulose, making it indigestible. Amylopectin, glycogen, and amylose are digestible starches with alpha linkages.
A proton has a ________ charge?
- Negative
- Positive
- Neutral
- None
Explanation: Answer reason: A proton carries a +1 elementary charge; electrons are negative, and neutrons are neutral.
An enzyme that catalyses the conversion of an aldose sugar to a ketose sugar would be classified as one of the following?
- Transferases
- Isomerases
- Oxidoreductases
- Hydrolase
- Lyases
Explanation: Answer reason: Aldose-to-ketose conversion is an intramolecular rearrangement (isomerization); enzymes that catalyze it are isomerases, e.g., aldose–ketose isomerases.
Where is glycogen stored?
- In the kidney and the liver
- In the stomach and kidney.
- In the muscle and the liver
- In the muscle and the stomach.
Explanation: Answer reason: Glycogen is stored primarily in the liver and skeletal muscle; the stomach and kidneys are not major glycogen stores.
What is the main product of protein metabolism?
- Iodine
- Carbohydrates
- Urea
- Glycogen
Explanation: Answer reason: Amino acid deamination generates ammonia, which is converted in the liver to urea—the principal nitrogenous waste of protein metabolism.
Which type of enzyme brings about intermolecular rearrangement of atoms in molecules?
- Lyases
- Hydrolases
- Isomerases
- Transference enzymes
Explanation: Answer reason: Isomerases catalyze intramolecular rearrangements, converting a molecule into one of its isomers. Lyases add or remove groups to form double bonds, hydrolases catalyze hydrolysis, and transfer enzymes move groups between molecules.
An enzyme increases the rate of a reaction by ........ activation energy?
- Increase
- Decrease
- Doesn't disturb.
- None of these.
Explanation: Answer reason: Enzymes accelerate reactions by lowering the activation energy, typically by stabilizing the transition state.
A positive Benedict's test is not given by?
- Sucrose
- Lactose
- Maltose
- Glucose
Explanation: Answer reason: Benedict’s test detects reducing sugars. Glucose, lactose, and maltose are reducing sugars, while sucrose is nonreducing because both anomeric carbons are involved in its glycosidic bond.
An alpha helix is formed where the highest probability of ........ sequence is present?
- Male
- Make
- Made
- Fake
Explanation: Answer reason: Alpha-helical segments are favored by the amino acids methionine, alanine, leucine, glutamate, and lysine (MALEK). Among the choices, MALE best represents this high helix-forming propensity.
The primary structure of a protein is formed by?
- Hydrogen bonds
- Peptide bonds
- Disulfide bonds
- All of these.
Explanation: Answer reason: The primary structure is the linear sequence of amino acids linked by peptide bonds; hydrogen bonds and disulfide bonds stabilize higher-order structures.
The most important epimers of glucose are?
- Galactose
- Fructose
- Arabinose
- Xylose
Explanation: Answer reason: Glucose and galactose differ in configuration at C-4, making galactose the key epimer of glucose; fructose is a ketose, and arabinose and xylose are pentoses.
Α-D-glucose and β-D-glucose are related by?
- Epimers
- Anomers
- Multirotation
- Keto-enol pair
Explanation: Answer reason: α- and β-D-glucose are cyclic forms that differ only in the configuration at the anomeric carbon (C1), making them anomers.
Account for the net production of ATP at each step of cellular respiration for one molecule of glucose?
- Glycolysis = 2, citric acid cycle = 2, oxidative phosphorylation = 32
- Glycolysis = 4, citric acid cycle = 2, oxidative phosphorylation = 32
- Glycolysis = 2, citric acid cycle = 1, oxidative phosphorylation = 32
- Glycolysis = 2, citric acid cycle = 2, oxidative phosphorylation = 36
Explanation: Answer reason: Net ATP per glucose: glycolysis yields 2 ATP, the citric acid cycle yields 2 ATP (as GTP), and oxidative phosphorylation contributes about 32 ATP, totaling ~36. This matches option A.
What is the energy currency of the cell called?
- RNA
- ADP
- ATP
- DNA
Explanation: Answer reason: ATP (adenosine triphosphate) stores and transfers energy for cellular processes; ADP has one fewer phosphate and less energy, and RNA and DNA are nucleic acids, not primary energy carriers.
Sickle cell anemia is due to the replacement of glutamic acid with which amino acid?
- Leucine
- Glycine
- Valine
- Isoleucine
Explanation: Answer reason: In sickle cell disease, a missense mutation in the β-globin gene substitutes nonpolar valine for negatively charged glutamic acid at position 6 of the β chain.
All proteins contain the?
- Same 20 amino acids.
- Different amino acids.
- 300 amino acids occurring in nature
- Only a few amino acids
Explanation: Answer reason: Proteins are polymers built from the common set of 20 standard amino acids, even though many other amino acids exist in nature.
The carbohydrate of the blood group substances is?
- Sucrose
- Fucose
- Arabinose
- Maltose
Explanation: Answer reason: ABO blood group antigens are oligosaccharides that characteristically contain L-fucose as a terminal sugar.
A polysaccharide that is often called animal starch is?
- Glycogen
- Starch
- Inulin
- Dextrin
Explanation: Answer reason: Glycogen is the storage polysaccharide in animals, commonly called animal starch, stored mainly in the liver and muscles.
C-O-C is a functional group of _____________?
- Alkane
- Alcohol
- Ether
- Ketone
Explanation: Answer reason: The ether functional group consists of an oxygen atom bonded to two carbon atoms (C–O–C).
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