Histology Practice Test 2
Histology NCLEX Practice Test
Histology is a key topic within the NCLEX test plan, located under Nursing Science → Clinical Foundations → Histology. This section relates tissue structure to healing, disease recognition, and wound care principles. Each test contains 50 questions designed to mirror the difficulty and variety of the real exam.
This is the 2nd part of the Histology series. To explore all practice tests under this topic, use the “Back to Main Topic” button at the end of the page.
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In the Histology Study Cards section, shared by real NCLEX candidates, you’ll find concise summaries and high-yield insights related to the most tested concepts. It’s a perfect space to reinforce challenging topics and sharpen your recall through quick, focused repetitions. Short, powerful, and repeatable!
Histology Practice Test 2
What is the watery fluid of a cell in which many organelles including the nucleus are found called?
- Cytoplasm
- Nucleoplasm
- Cytosol
- Chromoplast
Explanation: Answer reason: Cytoplasm is the gel-like material of the cell in which organelles are suspended. Nucleoplasm is only inside the nucleus, cytosol is just the liquid portion of cytoplasm, and chromoplasts are plant pigments.
What type of epithelial tissue is found in the uterine tubes?
- Squamous
- Ciliated
- Columnar
- Simple
Explanation: Answer reason: The uterine (fallopian) tubes are lined by simple ciliated columnar epithelium; the cilia help move the oocyte toward the uterus. Among the choices, 'Ciliated' best identifies this epithelial type.
Structure and motility of the cell is maintained by which of the following?
- Cartilage
- Microtubules
- Nucleus
- Gobi apparatus
Explanation: Answer reason: Microtubules are a major component of the cytoskeleton, providing structural support and enabling cell motility (e.g., cilia and flagella).
Which option represents the development of the structure of complexity in a human being?
- Organ
- Tissue
- Human being
- System
Explanation: Answer reason: Biological organization progresses from cells to tissues to organs to systems to the organism. Among the options, tissue is the earliest structural level indicating the development of complexity.
Which of the following cell organelles is responsible for protein synthesis?
- Mitochondria
- Ribosomes
- Golgi Apparatus
- Lysosomes
Explanation: Answer reason: Ribosomes translate mRNA into polypeptide chains. Mitochondria produce ATP, the Golgi modifies and packages proteins, and lysosomes digest cellular waste.
Where are peg cells present?
- Ampulla
- Isthmus
Explanation: Answer reason: Peg cells are non-ciliated secretory cells of the uterine tube and are most abundant in the ampulla, providing nutritive secretions for the ovum; they are sparse in the isthmus.
What type of cell is a peg cell that protects the ovum or sperm?
- Ovum
- Sperm
Explanation: Answer reason: Peg cells are secretory epithelial cells of the uterine tube that secrete nutritive, protective fluid primarily for the ovum (and early zygote).
What is the protein factory in a human cell?
- Ribosome
- Nucleus
- Cytoplasm
- Golgi apparatus
Explanation: Answer reason: Ribosomes are the cellular organelles that synthesize proteins; hence they are known as the protein factories of the cell.
Blood is classified as which of the following?
- Tissue
- Organ
- Cell
Explanation: Answer reason: Blood is a connective tissue composed of cells suspended in plasma, so it is classified as tissue rather than an organ or a single cell.
Which organelle is responsible for energy production in the cell?
- Smooth endoplasmic reticulum
- Mitochondrion
- Vacuole
- Golgi apparatus
- Nuclear membrane
Explanation: Answer reason: Mitochondria generate cellular ATP via oxidative phosphorylation, serving as the cell’s primary energy producers.
What type of epithelium lines the fallopian tube?
- Columnar epithelium
- Stratified epithelium
- Ciliated epithelium
- Germinal epithelium
Explanation: Answer reason: The uterine (fallopian) tube is lined by simple columnar ciliated epithelium with cilia that help move the ovum toward the uterus.
The power house of the cell is?
- Nucleus
- Cell membrane
- Mitochondria
- Lysosomes
Explanation: Answer reason: Mitochondria generate most cellular ATP via oxidative phosphorylation, hence called the cell’s power house.
What is the secretion of a goblet cell known as?
- Hormone
- Mucous
- Enzyme
- Digestive juice
Explanation: Answer reason: Goblet cells are unicellular mucous-secreting epithelial cells; their product is mucus, not hormones, enzymes, or digestive juices.
In which body structure is keratinized stratified squamous epithelium found?
- Conjunctiva of the eyes
- Hair
- Airway
- Stomach
Explanation: Answer reason: Keratinized stratified squamous epithelium is a durable, protective tissue found on surfaces requiring resistance to friction and dehydration, including the epidermis and associated structures such as hair follicles. Hair shaft formation depends on keratinized epithelial cells.
Suicide Bags are also called?
- Golgi Bodies
- Lysosomes
- Ribosomes
- Chromoplasts
Explanation: Answer reason: Lysosomes contain numerous hydrolytic enzymes that digest cellular debris and can trigger autolysis if they rupture, hence the nickname 'suicide bags.' They are key organelles for intracellular digestion and autophagy. Golgi bodies process and package proteins, ribosomes synthesize proteins, and chromoplasts are plant plastids for pigment storage, none of which mediate cellular self-digestion.
Observation in an interphase nucleus under the light microscope
- Chromosomes
- Nucleosomes
- Centromere
- Heterochromatin
Explanation: Answer reason: In interphase, chromatin is dispersed, with dense clumps of heterochromatin that stain darkly and are visible by light microscopy. Individual chromosomes and centromeres are not distinguishable until mitosis. Nucleosomes are submicroscopic (≈11 nm) and require electron microscopy. Therefore, the observable structure is heterochromatin.
Blood is?
- Cell
- Tissue
- Organ
- None of these
Explanation: Answer reason: Blood is a specialized connective tissue composed of formed elements (erythrocytes, leukocytes, platelets) suspended in a fluid extracellular matrix (plasma). It functions as a unit to transport gases, nutrients, wastes, and signaling molecules, fulfilling the definition of tissue rather than a single cell. It is not an organ because it lacks organized parenchyma and stroma forming a discrete structure.
An example for fibrocartilage is?
- Costal cartilage
- Pinna of ear
- Intervertebral disc
- Epiglottis
Explanation: Answer reason: Fibrocartilage is rich in type I collagen, providing high tensile strength and resistance to compression and shear. Classic locations include the intervertebral discs (especially the annulus fibrosus), pubic symphysis, and menisci. Costal cartilage is hyaline cartilage, while the pinna of the ear and the epiglottis are elastic cartilage. Therefore, the intervertebral disc is the correct example of fibrocartilage.
Wright stain is a...?
- Acidic stain
- Basic stain
- Neutral stain
- Supravital stain
Explanation: Answer reason: Wright stain is a Romanowsky-type neutral stain composed of both an acidic dye (eosin Y) and a basic dye (methylene blue/azure). The mixture forms neutral complexes that differentially color cellular components, staining acidic elements like cytoplasm with eosin and basophilic structures like nuclei with methylene blue. It is widely used for peripheral blood and bone marrow smears to distinguish leukocyte types and other formed elements.
Blood is a?
- Epithelial tissue
- Mascular tissue
- Connective tissue
- Nervous tissue
Explanation: Answer reason: Blood is classified as a connective tissue because it consists of cells (erythrocytes, leukocytes, platelets) suspended in an extracellular matrix, the plasma. Like other connective tissues, it is derived from mesenchyme and functions in transport, protection, and regulation. The presence of abundant matrix relative to cells is a defining histologic feature of connective tissues.
The epithelial membrane that surrounds the heart and lungs separately is called a .......... membrane.?
- Meninges
- Cutaneous
- Serous
- Synovial
Explanation: Answer reason: The pericardium around the heart and the pleura around the lungs are serous membranes. Serous membranes are epithelial-lined membranes that line closed body cavities and secrete serous fluid to reduce friction. Meninges cover the brain and spinal cord, cutaneous membrane is the skin, and synovial membranes line joint cavities.
The longest cell in human body is?
- Neuron
- Muscle fibre
- Bone cell
Explanation: Answer reason: Neurons can extend a single axon from the spinal cord to the foot, making some motor neurons nearly 1 meter long, the longest single cells in the body. Skeletal muscle fibres can be long (up to tens of centimeters) but are generally shorter than the longest neurons. Bone cells such as osteocytes are comparatively small. Therefore, neuron is the best answer.
Which of the following statements is mis-matching?
- Cellular boundary - Cellular membrane
- Cell control center - Nucleus
- Cell assembly line - Cytoplasm
- Cell controls - Storage area
Explanation: Answer reason: The plasma membrane forms the cellular boundary, and the nucleus functions as the cell’s control center. The cytoplasm is the site of many biosynthetic reactions and can be likened to an assembly area. However, “storage area” describes vacuoles/vesicles, not cell control; control of cellular activities is mediated by the nucleus. Therefore, the pair “Cell controls – Storage area” is mismatched.
The epithelial tissue found lining the urinary bladder is -?
- Sqamous
- Columnar
- Ciliated
- Transitional
Explanation: Answer reason: The urinary bladder is lined by transitional epithelium (urothelium), a stratified epithelium specialized to stretch and recoil as the bladder fills and empties. Its dome-shaped umbrella cells protect underlying tissues from the hypertonic and potentially toxic effects of urine. Squamous, columnar, and ciliated epithelia do not provide this distensibility and barrier function characteristic of the bladder lining.
Which of the following plant shows multi-epidermis?
- Croton
- Allium
- Nerium
- Cucurbita
Explanation: Answer reason: Nerium (oleander) leaves are xeromorphic and characteristically possess a multiseriate epidermis, which helps reduce water loss. The epidermis may be two or more layers thick with thick cuticle and sunken stomata. Croton, Allium, and Cucurbita typically have a single-layered epidermis in their leaves.
Kupffer cells are present in?
- Bone
- Brain
- Liver
- Lung
Explanation: Answer reason: Kupffer cells are the resident macrophages of the liver, lining the hepatic sinusoids. They are part of the mononuclear phagocyte system and clear bacteria, debris, and endotoxin from portal blood. In contrast, analogous cells in bone are osteoclasts, in brain are microglia, and in lung are alveolar macrophages.
Engine of cell ??
- Ribosome
- Mitochondria
- Golgi appartus
- Lysosome
Explanation: Answer reason: Mitochondria generate ATP via oxidative phosphorylation, supplying the cell’s energy and thus are considered its engine or powerhouse. Ribosomes primarily synthesize proteins. The Golgi apparatus modifies and packages proteins and lipids. Lysosomes are involved in intracellular digestion, not energy production.
Which of the following is not a connective tissue?
- Bone
- Muscles
- Blood
- Cartilages
Explanation: Answer reason: Bone, cartilage, and blood are recognized types of connective tissue, characterized by cells embedded in an extracellular matrix. Muscle tissue, however, is a distinct primary tissue type specialized for contraction and movement. It does not function primarily in support or matrix production like connective tissues. Therefore, muscles are not a connective tissue.
In which of the following type of cells the Gap junctions are absent?
- Sperm cells
- Brain cells
- Reproductive cells
- Cardiac cells
Explanation: Answer reason: Gap junctions (connexin-based channels) enable intercellular electrical and metabolic coupling, prominent in cardiac muscle and among many neural and glial cells. Oocytes also maintain gap junctions with surrounding granulosa cells. Sperm are solitary, motile gametes that do not form stable intercellular junctions with other sperm, so gap junctions are absent. Therefore, sperm cells are the best answer.
The epithelial tissue which is found in uterine tubes is-?
- Squamous
- Ciliated
- Columnar
- Simple
Explanation: Answer reason: The uterine (fallopian) tubes are lined by simple ciliated columnar epithelium. The cilia beat toward the uterus, helping transport the oocyte or zygote. Secretory peg cells are also present, but the defining feature is the ciliation. Among the given options, 'Ciliated' best identifies this epithelial type.
The cell contents excluding the nucleus is known as?
- Cytosol
- Cytoplasm
- Chromosomes
- Chromatin
Explanation: Answer reason: Cytoplasm refers to all cellular material outside the nucleus, including cytosol and organelles. Cytosol is only the fluid portion and excludes membrane-bound organelles. Chromosomes and chromatin are nuclear contents. Therefore, the best answer is cytoplasm.
Histology is the study of?
- Cells
- Tissues
- Organs
- Bones
Explanation: Answer reason: Histology is the microscopic study of tissues, examining how cells are organized into tissue types and their structure-function relationships. Cytology refers to the study of individual cells, while organology concerns whole organs. Osteology specifically studies bones, a subset of anatomy rather than histology. Therefore, the correct choice is tissues.
The study of tissue?
- Zoology
- Cytology
- Histology
Explanation: Answer reason: Histology is the branch of biology that studies tissues and their microscopic structure. Cytology focuses on individual cells, not tissues. Zoology is the study of animals as whole organisms. Therefore, the study of tissues is histology.
The power house of a cell is-?
- Ribosomes
- Lysosomes
- Mitochondira
- Granules
Explanation: Answer reason: Mitochondria generate most of the cell’s ATP through oxidative phosphorylation, providing the energy required for cellular processes, hence termed the cell’s powerhouse. Ribosomes are sites of protein synthesis, not primary energy production. Lysosomes are responsible for intracellular digestion. Granules generally store substances and do not produce ATP.
The study of tissues is known as?
- Cytology
- Histology
- Anatomy
- Embryology
Explanation: Answer reason: Histology is the branch of biology that studies tissues and their microscopic structure. Cytology focuses on individual cells, anatomy studies the macroscopic structure of the body and organs, and embryology deals with development from conception to birth. Therefore, the correct term for the study of tissues is histology.
The most abundant tissue in the human body is?
- Epithelial tissue
- Connective tissue
- Nervous tissue
- Muscle tissue
Explanation: Answer reason: Connective tissue is the most abundant and widely distributed tissue type in the body. It includes diverse forms such as bone, cartilage, adipose tissue, blood, and tendons, which collectively occupy a large proportion of body mass and provide support, protection, and transport. Epithelial, muscle, and nervous tissues are extensive but not as widespread in variety and volume as connective tissues.
Which fixative is commonly used in histopathology?
- Ethanol
- Formalin
- Acetone
- Osmium tetroxide
Explanation: Answer reason: Neutral buffered formalin (10% formalin) is the standard routine fixative in histopathology because it cross-links proteins, preserves tissue architecture, and is compatible with paraffin processing and H&E staining. Ethanol and acetone are coagulative fixatives used more for cytology or frozen sections and can cause tissue shrinkage. Osmium tetroxide is mainly used as a secondary fixative in electron microscopy for lipid preservation, not for routine histology.
Blood is classified as which type of tissue?
- Epithelial tissue
- Connective tissue
- Muscular tissue
- Nervous tissue
Explanation: Answer reason: Blood is a specialized connective tissue with a liquid extracellular matrix (plasma) containing proteins and dissolved substances. Its formed elements—erythrocytes, leukocytes, and platelets—are suspended in this matrix, fitting the connective tissue definition of cells embedded within an extracellular matrix. Developmentally, blood arises from mesenchyme like other connective tissues. Its roles in transport, defense, and hemostasis further distinguish it from epithelial, muscle, or nervous tissues.
Lysosomes are abundantly found in?
- Phagocytic WBC
- Nerve cells
- Muscle cells
- None of these
Explanation: Answer reason: Lysosomes contain hydrolytic enzymes for intracellular digestion. Phagocytic white blood cells such as neutrophils and macrophages ingest microbes and debris, requiring numerous lysosomes to fuse with phagosomes and degrade contents. Neurons and muscle cells are not primarily phagocytic and have comparatively fewer lysosomes. Therefore, lysosomes are abundant in phagocytic WBCs.
Chromosome are Presents in?
- Nucleus
- Cytoplasm
- Lysosome
- Golgi
Explanation: Answer reason: In eukaryotic cells, chromosomes are DNA–protein complexes localized within the nucleus, where genetic material is organized as chromatin and condenses during cell division. The cytoplasm contains organelles but not nuclear chromosomes. Lysosomes are digestive organelles, and the Golgi apparatus modifies and packages proteins; neither houses chromosomes. Therefore, the correct location is the nucleus.
Which part of liver stores Vitamin A?
- Ito cells
- Hepatocyte
- Kupffer cells
- Endothelial cells
Explanation: Answer reason: Vitamin A is stored in the liver primarily in hepatic stellate cells (Ito cells) located in the space of Disse, where it is kept as retinyl esters within lipid droplets. Hepatocytes perform many metabolic and synthetic functions but are not the principal storage site for vitamin A. Kupffer cells are liver macrophages for phagocytosis and immune surveillance, and sinusoidal endothelial cells line the sinusoids; neither is specialized for vitamin A storage.
When did the fluid mosaic model proposed?
- 1977
- 1968
- 1975
- 1972
Explanation: Answer reason: The fluid mosaic model of the cell membrane was proposed by Singer and Nicolson in 1972. describes a phospholipid bilayer with proteins embedded and able to move laterally, explaining membrane flexibility and function. The other years listed do not correspond to the original proposal date of this foundational membrane model.
Study of Tissue is called?
- Neurology
- Histology
- Hepatology
- Cardiology
Explanation: Answer reason: Histology is the branch of biology/medicine that studies tissues, typically by examining their microscopic structure and organization. Neurology focuses on the nervous system, hepatology on the liver, and cardiology on the heart. Therefore, the correct term for the study of tissues is histology.
Resistance to tension is the main function of?
- Collagen type2
- Collagen type7
- Collagen type1
- Collagen type3
Explanation: Answer reason: Type I collagen is the predominant collagen in tissues designed to withstand tensile (pulling) forces, such as tendons, ligaments, bone, and dermis. Its thick, densely packed fibers provide high tensile strength. By contrast, type II collagen is mainly for cartilage (compression resistance), type III is reticular/supportive in extensible organs and early wound healing, and type VII forms anchoring fibrils at the dermal-epidermal junction.
Microtubules Consist of helically stacked molecules of the protein Called?
- Actin
- Myosin
- Tubulin
- Keratin
Explanation: Answer reason: Microtubules are cytoskeletal structures built from α- and β-tubulin heterodimers that polymerize into protofilaments and assemble into a hollow tube. These tubulin subunits are arranged in a helical lattice, giving microtubules their characteristic structure and polarity. Actin forms microfilaments, keratin forms intermediate filaments, and myosin is a motor protein that interacts with actin rather than forming microtubules.
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